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Literature summary extracted from

  • Ghosal, A.; Banas, A.; Stahl, U.; Dahlqvist, A.; Lindqvist, Y.; Stymne, S.
    Saccharomyces cerevisiae phospholipid:diacylglycerol acyl transferase (PDAT) devoid of its membrane anchor region is a soluble and active enzyme retaining its substrate specificities (2007), Biochim. Biophys. Acta, 1771, 1457-1463.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.158 molecular biology the conversion of a membrane bound lipid metabolizing enzyme into a soluble and active form might be applied to other enzymes with a membrane anchor region. Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.158 expression in Escherichia coli, expression in Pichia pastoris of a N-terminal deleted version of PDAT, lacking the predicted membrane-spanning region under the control of the methanol inducible alcohol oxidase promoter Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.158 membrane soluble form is produced for comparison with the native membrane bound enzyme Saccharomyces cerevisiae 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.158 67000
-
 SDS-PAGE, after deglycosylation Saccharomyces cerevisiae
2.3.1.158 80000
-
 SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.158 Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.158 glycoprotein extensive deglycosylation severely decreases the activity Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.158 the recombinant protein by Ni2+ affinity chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.158 0.00138
-
 sn-1,2-distearoylphosphatidylethanolamine as substrate using the membrane bound enzyme Saccharomyces cerevisiae
2.3.1.158 0.001399
-
 sn-1,2-dipalmitoylphosphatidylethanolamine as substrate using the membrane bound enzyme Saccharomyces cerevisiae
2.3.1.158 0.002453
-
 sn-1,2-dilinolenoylphosphatidylethanolamine as substrate using the membrane bound enzyme Saccharomyces cerevisiae
2.3.1.158 0.002468
-
 sn-1,2-dioleoylphosphatidylethanolamine as substrate using the membrane bound enzyme Saccharomyces cerevisiae
2.3.1.158 0.002597
-
 sn-1,2-dilinoleoylphosphatidylethanolamine as substrate using the membrane bound enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.158 rac-1,2-divernoleoylglycerol + 1,3-dioleoyl-sn-glycerol
-
 Saccharomyces cerevisiae 1-oleoyl-sn-glycerol + rac-1,2-divernoleoyl-3-oleoylglycerol
-
 ?
2.3.1.158 sn-1,2-dioleoylglycerol + sn-1,2-dioleoylglycerol
-
 Saccharomyces cerevisiae trioleoylglycerol + sn-1-oleoylglycerol
-
 ?
2.3.1.158 sn-1,2-dioleoylphosphatidylcholine + sn-1,2-dioleoylglycerol
-
 Saccharomyces cerevisiae trioleoylglycerol + sn-1-oleoylphosphatidylcholine
-
 ?
2.3.1.158 sn-1,2-dioleoylphosphatidylcholine + sn-1-oleoylglycerol
-
 Saccharomyces cerevisiae ? + sn-1-oleoylphosphatidylcholine
-
 ?
2.3.1.158 sn-1,2-dioleoylphosphatidylcholine + sn-2-oleoylglycerol
-
 Saccharomyces cerevisiae ? + sn-1-oleoylphosphatidylcholine
-
 ?
2.3.1.158 sn-1,2-dioleoylphosphatidylethanolamine + sn-1,2-dioleoylglycerol
-
 Saccharomyces cerevisiae trioleoylglycerol + 1-oleoylphosphatidylethanolamine
-
 ?
2.3.1.158 sn-1,3-dioleoylglycerol + sn-1,3-dioleoylglycerol
-
 Saccharomyces cerevisiae trioleoylglycerol + sn-1-oleoylglycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.158 PDAT
-
 Saccharomyces cerevisiae
2.3.1.158 phospholipid:diacylglycerol acyltransferase
-
 Saccharomyces cerevisiae
2.3.1.158 ScPDAT
-
 Saccharomyces cerevisiae